FOX2 Antibody - BSA Free

Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588]

Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- usedat a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: FOX2 Antibody [NB110-40588]

Immunoprecipitation: FOX2 Antibody [NB110-40588] - Detection of human RBM9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody NB110-40588 (lot NB110-40588-2) used for IP at 6 ug/mg lysate. RBM9 was also immunoprecipitated by a previous lot (lot NB110-40588-1) of this antibody. For blotting immunoprecipitated RBM9, NB110-40588 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: FOX2 Antibody - BSA Free [NB110-40588] -

Rbfox2 specifically modulates CaV1.2 alternative exon 9* splicing in cardiomyocyte. (A) H9c2 cells were transfected with nontargeting (NT) or Rbfox2 siRNA for 48 h. The endogenous expression of Rbfox2 protein was detected by Western blotting, the beta -actin was detected as internal control. PCR product of CaV1.2E9* channel was amplified from cDNA libraries and separated on 2.5% agarose gel. Actb mRNA was detected as internal control. (B) The relative expression of Rbfox2 was normalized to beta -actin. (C) The value for percent CaV1.2E9* inclusion was the upper band intensity divided by the summed intensities of upper and lower bands, and presented as a bar chart. (D) H9c2 cells were transfected with an empty vector, WT Rbfox2, DN Rbfox2 or WT plus DN Rbfox2 expression plasmids, nontreated cells were set as negative control (NC). After 48 h incubation, the expression of Rbfox2 protein was detected by Western blotting, the beta -actin was detected as internal control. PCR products were separated on 2–3% agarose gel, which was used to check the proportions of CaV1.2E9* channels. (E) Relative expression of Rbfox2 was normalized to beta -actin. (F) The proportion of CaV1.2E9* channels were analyzed and presented by a bar chart. n = 3 independent experiments. *P < 0.05, **P < 0.01, one-way ANOVA followed by a Tukey’s post hoc test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37415128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FOX2 Antibody - BSA Free [NB110-40588] -

Rbfox2 specifically modulates CaV1.2 alternative exon 9* splicing in cardiomyocyte. (A) H9c2 cells were transfected with nontargeting (NT) or Rbfox2 siRNA for 48 h. The endogenous expression of Rbfox2 protein was detected by Western blotting, the beta -actin was detected as internal control. PCR product of CaV1.2E9* channel was amplified from cDNA libraries and separated on 2.5% agarose gel. Actb mRNA was detected as internal control. (B) The relative expression of Rbfox2 was normalized to beta -actin. (C) The value for percent CaV1.2E9* inclusion was the upper band intensity divided by the summed intensities of upper and lower bands, and presented as a bar chart. (D) H9c2 cells were transfected with an empty vector, WT Rbfox2, DN Rbfox2 or WT plus DN Rbfox2 expression plasmids, nontreated cells were set as negative control (NC). After 48 h incubation, the expression of Rbfox2 protein was detected by Western blotting, the beta -actin was detected as internal control. PCR products were separated on 2–3% agarose gel, which was used to check the proportions of CaV1.2E9* channels. (E) Relative expression of Rbfox2 was normalized to beta -actin. (F) The proportion of CaV1.2E9* channels were analyzed and presented by a bar chart. n = 3 independent experiments. *P < 0.05, **P < 0.01, one-way ANOVA followed by a Tukey’s post hoc test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37415128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FOX2 Antibody - BSA Free [NB110-40588] -

Knockdown of Rbfox2 hyperpolarizes window currents of CaV1.2 channel in NRVMs. (A) The protein expression of Rbfox2 and beta -actin were detected in whole-cell lysate of isolated NRVMs by using Western blotting after transfecting with NT or Rbfox2 siRNAs. The membrane protein was also extracted, and membrane expression of CaV1.2 alpha 1C was checked, Na-K ATPase was detected as a membrane loading control. (B) Relative expression level of Rbfox2 was normalized with beta -actin in differentially transfected cells, and presented as a bar chart. n = 4 independent experiments. **P < 0.001, one-way ANOVA followed by a Tukey’s post hoc test. (C) Relative CaV1.2 alpha 1C membrane expression was normalized with Na-K ATPase in differentially-transfected cells. n = 4 independent experiments. P = 0.6679, one-way ANOVA followed by a Tukey’s post hoc test. (D) Raw traces of CaV1.2 whole-cell calcium current recorded from NRVMs treated with NT or Rbfox2 siRNA in 10 mmol/L Ba2+ external solution. (E) I-V relationship of calcium channel current recorded under the different testing potential, increased from − 50 to 50 mV in NRVMs transfected with NT or Rbfox2 siRNA. (F) CaV1.2 channel current density in NRVMs was analyzed after transfected with NT or Rbfox2 siRNA. (H) Plots of steady-state activation (SSA) curve of CaV1.2 channel were analyzed from I-V currents in NT or Rbfox2 siRNA-treated NRVMs. (H) Plots of the steady-state inactivation (SSI) was also recorded in NRVMs. (I) CaV1.2 window currents were superimposed from SSI (f∞) and SSA (d∞) curves of NRVMs Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37415128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.