FOXG1 Antibody

Novus Biologicals | Catalog # NB100-1099

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FOXG1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HQNQGSSSNPLIH corresponding to C-Terminus according to NP_005240.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FOXG1 Antibody

FOXG1 Antibody

Western Blot: FOXG1 Antibody [NB100-1099] -

Western Blot: FOXG1 Antibody [NB100-1099] - (1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
FOXG1 Antibody

Western Blot: FOXG1 Antibody [NB100-1099] -

Western Blot: FOXG1 Antibody [NB100-1099] - (0.5ug/ml) staining of Human Cerebellum (A) and Cerebral Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
FOXG1 Antibody

Flow Cytometry: FOXG1 Antibody [NB100-1099] -

Flow Cytometry: FOXG1 Antibody [NB100-1099] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for FOXG1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 48kDa band observed in lysates of cell line U251, and approx. 48-50kDa in Human Cerebellum and Cerebral Cortex lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B), and 50.5kDa according to CAA55038.1 (FOXG1A). An additional band at 70-75kDa was observed in some lysates and was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FOXG1

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon. This gene participates in signal transduction Activin A signaling regulation, TGF-beta receptor signaling pathway, transcription factors in neurogenesis, as well as regulation of nuclear SMAD2/3 signaling. It is thought to interact with genes FOXO1, FOXO3, FOXO4, SMAD2, and SMAD3. FOXG1 is associated with diseases such as melanoma, neuronitis, glioblastoma, purpura, west syndrome, rett syndrome, medulloblastoma, hypotonia, retinitis, maternal uniparental disomy, chromosome 14, intellectual disabilities, brain malformations, multiple sclerosis, and lupus.

Alternate Names

BF-1, BF1HBF-2, BF2, BF-2, Brain factor 1, Brain factor 2, FHKL3, FKHL1forkhead-like 1, FKHL2, FKHL3, FKHL4, forkhead box G1, forkhead box G1A, forkhead box G1B, forkhead box G1C, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, forkhead-like 3, forkhead-like 4, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, FOXG1A, FOXG1Bforkhead-like 2, FOXG1C, HBF-1, hBF-2, HBF-3, HFK1HBF-G2, HFK2HBF2, HFK3KHL2, oncogene QIN, QINFKH2forkhead box protein G1

Entrez Gene IDs

2290 (Human); 15228 (Mouse); 24370 (Rat)

Gene Symbol

FOXG1

Additional FOXG1 Products

Product Documents for FOXG1 Antibody

Certificate of Analysis

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Product Specific Notices for FOXG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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