FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20155

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RAB-S249

Format

Azide and BSA Free
View all FoxP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained by recombinant antibody (rAb) phage display recognizing FOXP2 protein under non-denaturing conditions.

Epitope

This antibody binds to a folded domain, amino acids 306-411.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free

FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free [NBP3-20155] -

Immunocytochemistry/Immunofluorescence: FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free [NBP3-20155] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-S249 (NBP3-20155) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20155, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free

Flow Cytometry: FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free [NBP3-20155] -

Flow Cytometry: FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free [NBP3-20155] - HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-S249 (NBP3-20155, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: FoxP2

FOXP2 encodes an evolutionarily conserved transcription factor expressed in fetal and adult brain. This transcription factor is a member of the forkhead/winged-helix (FOX) family of transcription factors, and contains a FOX DNA-binding domain and a large polyglutamine tract. Members of the FOX family of transcription factors are regulators of embryogenesis. The product of this gene is thought to be required for proper development of speech and language regions of the brain during embryogenesis. Although a point mutation in this gene has been associated with the KE pedigree segregating developmental verbal dyspraxia, no association between mutations in this gene and another speech disorder, autism, has been found. Four alternative transcripts encoding three different isoforms have been identified.

Long Name

Forkhead Box P2

Alternate Names

CAGH44, SPCH1, TNRC10

Gene Symbol

FOXP2

Additional FoxP2 Products

Product Documents for FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FoxP2 Antibody (RAB-S249) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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