FPR1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56473

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Primate

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FPR1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 176-190 of human FPR1 (CTFNFSPWTNDPKER) was used as the immunogen

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FPR1 Antibody - BSA Free

Western Blot: FPR1 AntibodyBSA Free [NB100-56473]

Western Blot: FPR1 AntibodyBSA Free [NB100-56473]

Western Blot: FPR1 Antibody [NB100-56473] - Analysis of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using this antibody at 1 ug/ml.
Simple Western: FPR1 AntibodyBSA Free [NB100-56473]

Simple Western: FPR1 AntibodyBSA Free [NB100-56473]

Simple Western: FPR1 Antibody [NB100-56473] - Shows a specific band for FPR1 in 0.5 mg/ml of Mouse Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

Applications for FPR1 Antibody - BSA Free

Application
Recommended Usage

Simple Western

100 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse Liver lysate 0.5 mg/mL, separated by Size, antibody dilution of 100 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FPR1

FPR1 is a member of chemoattractant/chemokine GPCRs expressed mainly on leukocytes, neutrophils and monocytes. FPR1 is a seven transmembrane pertussis toxin-sensitive GPCR and binds to formylated peptides generated by bacteria resulting in leukocyte trafficking to the site of inflammation. Ligand binding to FPR results in activation of multiple effectors including phospholipase C leading to calcium mobilization and PKC activation. Other implications of FPR signaling include polarization of actin cytoskeleton, activation of various integrins, increased cell migration, phagocytosis, degranulation and release of reactive oxygen intermediates.

Long Name

Formyl Peptide Receptor 1

Alternate Names

fMLF-R, FMLP

Entrez Gene IDs

2357 (Human)

Gene Symbol

FPR1

UniProt

P21462 (Human)

Additional FPR1 Products

Product Documents for FPR1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FPR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FPR1 Antibody - BSA Free

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