FRG1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99204

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all FRG1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Mouse FRG1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for FRG1 Antibody - Azide and BSA Free

Western Blot: FRG1 Antibody [NBP2-99204]

Western Blot: FRG1 Antibody [NBP2-99204]

Western Blot: FRG1 Antibody [NBP2-99204] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:29 kDa
Immunoprecipitation: FRG1 Antibody [NBP2-99204]

Immunoprecipitation: FRG1 Antibody [NBP2-99204]

Immunoprecipitation: FRG1 Antibody [NBP2-99204] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg A549 Whole Cell Lysate2 uL anti-Frg1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Frg1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 38 kDa
FRG1 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: FRG1 Antibody [NBP2-99204] -

Immunocytochemistry/Immunofluorescence: FRG1 Antibody [NBP2-99204] - Immunofluorescence staining of FRG1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99204 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to nucleus.

Applications for FRG1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FRG1

FRG1 maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus.

Alternate Names

facioscapulohumeral muscular dystrophy region gene-1, FSG1FRG1A, FSHD region gene 1, FSHD region gene 1 protein, protein FRG1

Gene Symbol

FRG1

Additional FRG1 Products

Product Documents for FRG1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FRG1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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