Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Rat IgG2A Clone # 163842
View all Frizzled-8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-8
Ala28-Pro173 (predicted)
Accession # Q61091

Specificity

Detects mouse Frizzled-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) Frizzled-6 is observed and no cross-reactivity with rmFrizzled-1, -2, -3, -4, or -7 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Frizzled-8 Antibody (163842) [Janelia Fluor® 549]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Frizzled-8

"Frizzled-8 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Alternate Names

Frizzled8, FZD8

Gene Symbol

FZD8

Additional Frizzled-8 Products

Product Documents for Frizzled-8 Antibody (163842) [Janelia Fluor® 549]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Frizzled-8 Antibody (163842) [Janelia Fluor® 549]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Family
Wnt Receptors

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