Human Frizzled-8 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-00385

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human Frizzled-8 in samples. No significant cross-reactivity or interference between Human Frizzled-8 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 10.09%

Inter-Assay Precision (Precision between assays) 9.95%

Recovery for Human Frizzled-8 ELISA Kit (Colorimetric)

Recovery

83 - 106%

Linearity

Human Frizzled-8 ELISA Kit (Colorimetric)

Scientific Data Images for Human Frizzled-8 ELISA Kit (Colorimetric)

Human Frizzled-8 ELISA Kit (Colorimetric)

ELISA: Human Frizzled-8 ELISA Kit (Colorimetric) [NBP3-00385] -

ELISA: Human Frizzled-8 ELISA Kit (Colorimetric) [NBP3-00385] - Standard Curve Reference

Kit Contents for Human Frizzled-8 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Frizzled-8

"Frizzled-8 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Alternate Names

Frizzled8, FZD8

Gene Symbol

FZD8

Additional Frizzled-8 Products

Product Documents for Human Frizzled-8 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Frizzled-8 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Wnt Family
Wnt Receptors

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