FUNDC1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-18250
![Western Blot: FUNDC1 Antibody [NBP3-18250]](https://resources.rndsystems.com/images/products/FUNDC1%20Antibody-Western%20Blot-NBP3-18250-img0002.jpg "Western Blot: FUNDC1 Antibody [NBP3-18250]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptide from the N-terminal of Human FUNDC1
Localization
Mitochondrion Outer Membrane|Multi-Pass Membrane Protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FUNDC1 Antibody - BSA Free
Western Blot: FUNDC1 Antibody [NBP3-18250]
Western Blot: FUNDC1 Antibody [NBP3-18250] - Western blot analysis of Mouse, Rat Liver cell lysates showing detection of ~17.2 kDa FUNDC1 protein using Rabbit Anti-FUNDC1 Polyclonal Antibody (NBP3-18250). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Lane 3: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody (NBP3-18250) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~17.2 kDa.![Immunohistochemistry: FUNDC1 Antibody [NBP3-18250]](https://resources.rndsystems.com/images/products/FUNDC1-Antibody-Immunohistochemistry-NBP3-18250-img0001.jpg "Immunohistochemistry: FUNDC1 Antibody [NBP3-18250]")
Immunohistochemistry: FUNDC1 Antibody [NBP3-18250]
Immunohistochemistry: FUNDC1 Antibody [NBP3-18250] - Immunohistochemistry analysis using Rabbit Anti-FUNDC1 Polyclonal Antibody (NBP3-18250). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody (NBP3-18250) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.Applications for FUNDC1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50
Western Blot
1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS, 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FUNDC1
Alternate Names
FUN14 domain containing 1, FUN14 domain-containing protein 1, MGC51029
Gene Symbol
FUNDC1
Additional FUNDC1 Products
Product Documents for FUNDC1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FUNDC1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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