FXYD3 Antibody (OTI4E3)

Novus Biologicals | Catalog # NBP2-01991

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4E3
View all FXYD3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FXYD3(NP_005962) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

7.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FXYD3 Antibody (OTI4E3)

Western Blot: FXYD3 Antibody (4E3) [NBP2-01991] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-FXYD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunocytochemistry/ Immunofluorescence: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunocytochemistry/Immunofluorescence: FXYD3 Antibody (4E3) [NBP2-01991] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FXYD3.
Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (4E3) [NBP2-01991] - Staining of paraffin-embedded Human prostate tissue using anti-FXYD3 mouse monoclonal antibody.
Western Blot: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Western Blot: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Western Blot: FXYD3 Antibody (4E3) [NBP2-01991] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FXYD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FXYD3.
Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (4E3) [NBP2-01991] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FXYD3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (4E3) [NBP2-01991] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FXYD3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (4E3) [NBP2-01991] - Staining of paraffin-embedded Human bladder tissue using anti-FXYD3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (4E3) [NBP2-01991] - Staining of paraffin-embedded Human colon tissue using anti-FXYD3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (OTI4E3) [NBP2-01991]

Immunohistochemistry-Paraffin: FXYD3 Antibody (4E3) [NBP2-01991] - Staining of paraffin-embedded Human liver tissue using anti-FXYD3 mouse monoclonal antibody.

Applications for FXYD3 Antibody (OTI4E3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1.11 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FXYD3

This gene belongs to a small family of FXYD-domain containing regulators of Na+/K+ ATPases which share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD, and containing 7 invariant and 6 highly conserved amino acids. This gene encodes a cell membrane protein that may regulate the function of ion-pumps and ion-channels. This gene may also play a role in tumor progression. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Alternate Names

Chloride conductance inducer protein Mat-8, FXYD domain containing ion transport regulator 3, FXYD domain-containing ion transport regulator 3, Mammary tumor 8 kDa protein, MAT8MAT-8, Phospholemman-like, phospholemman-like protein, PLMLMGC111076

Entrez Gene IDs

5349 (Human)

Gene Symbol

FXYD3

Additional FXYD3 Products

Product Documents for FXYD3 Antibody (OTI4E3)

Certificate of Analysis

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Product Specific Notices for FXYD3 Antibody (OTI4E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FXYD3 Antibody (OTI4E3)

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Protocols

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