G protein alpha inhibitor 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-59000

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all G protein alpha inhibitor 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ARQLFVLAGAAEEGFMTAELAGVIKR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G protein alpha inhibitor 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: G protein alpha inhibitor 1 Antibody [NBP2-59000]

Immunocytochemistry/ Immunofluorescence: G protein alpha inhibitor 1 Antibody [NBP2-59000]

Immunocytochemistry/Immunofluorescence: G protein alpha inhibitor 1 Antibody [NBP2-59000] - Staining of human cell line A-431 shows localization to centrosome.
G protein alpha inhibitor 1 Antibody

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -Staining of human cerebral cortex shows moderate membranous-cytoplasmic positivity in neuropil and astrocytes.
G protein alpha inhibitor 1 Antibody

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -Staining of human kidney shows strong membranous-cytoplasmic positivity in cells in tubules.
G protein alpha inhibitor 1 Antibody

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -Staining of human pancreas shows moderate membranous/cytoplasmic positivity in endocrine glandular cells.
G protein alpha inhibitor 1 Antibody

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] -

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody [NBP2-59000] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Applications for G protein alpha inhibitor 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC, Retrieval method: HIER pH6.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G protein alpha inhibitor 1

Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]

Alternate Names

Adenylate cyclase-inhibiting G alpha protein, Gi, Gi1 protein alpha subunit, guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 1, guanine nucleotide-binding protein G(i) subunit alpha-1

Gene Symbol

GNAI1

Additional G protein alpha inhibitor 1 Products

Product Documents for G protein alpha inhibitor 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for G protein alpha inhibitor 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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