G3BP1 Antibody

Novus Biologicals | Catalog # NBP1-18922

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Mammal

Cited:

Human, Mammal

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human GTPase activating protein (SH3 domain) binding protein 1 using the numbering given in entry NP_005745.1 (GeneID 10146).

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 28794026).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G3BP1 Antibody

Western Blot: G3BP1 Antibody [NBP1-18922]

Western Blot: G3BP1 Antibody [NBP1-18922]

Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate (50 ug) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second.
Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody [NBP1-18922]

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody [NBP1-18922]

Immunocytochemistry/Immunofluorescence: G3BP1 Antibody [NBP1-18922] - G3BP1 in red (Alexa fluor 555) and nucleus in blue (DAPI). Human primary fibroblasts untreated in the left and treated in the right with G3BP1 stress granules labeling. Alexa fluor 555 1s exposure and DAPI 1s exposure. Fixation: PFA 4% 10min RT, permeabilization: Triton 0.1% TBS, blocking: Donkey Serum 10% TBS-T, Ab dilution: 1:1000, Secondary Ab: Alexa fluor 555 Donkey anti-rabbit 1:600, Mounting medium with DAPI. Image submitted by a verified customer review.
Western Blot: G3BP1 Antibody [NBP1-18922]

Western Blot: G3BP1 Antibody [NBP1-18922]

Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Affinity purified rabbit anti-G3BP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. G3BP1 was also immunoprecipitated by rabbit anti-G3BP1 antibody NBP1-18923, which recognizes a downstream epitope.

Applications for G3BP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Validated from a verified customer review.

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP1-18922 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: G3BP1

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Alternate Names

ATP-dependent DNA helicase VIII, EC 3.6.1, EC 3.6.4.12, EC 3.6.4.13, G3BP-1, G3BPRas-GTPase-activating protein SH3-domain-binding protein, GAP binding protein, GAP SH3 domain-binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, hDH VIII, HDH-VIII, MGC111040, ras GTPase-activating protein-binding protein 1, RasGAP-associated endoribonuclease G3BP

Entrez Gene IDs

10146 (Human); 27041 (Mouse)

Gene Symbol

G3BP1

UniProt

Q13283 (Human)

Additional G3BP1 Products

Product Documents for G3BP1 Antibody

Certificate of Analysis

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Product Specific Notices for G3BP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for G3BP1 Antibody (1)

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1 Customer Rating
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Customer Images

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  • G3BP1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human primary fibroblast
    Species: Human
    Verified Customer | Posted 02/12/2019
    G3BP1 in red (Alexa fluor 555) and nucleus in blue (DAPI). Human primary fibroblasts untreated in the left and treated in the right with G3BP1 stress granules labeling. Alexa fluor 555 1s exposure and DAPI 1s exposure.
    ICC-IF Fixation: PFA 4% 10min RT permeabilization: Triton 0.1% TBS blocking: Donkey Serum 10% TBS-T 1. Ab dilution: 1:1000 Secondary Ab: Alexa fluor 555 Donkey anti-rabbit 1:600 Mounting medium with DAPI
    G3BP1 Antibody NBP1-18922

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Protocols

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FAQs

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