Detection of GABAA R δ N-Terminus by Western Blot. Western blot of mouse cerebellar lysates from wild type (Control) and δ knockout (KO) animals showing specific immunolabeling of the ~52 kDa δ subunit of the GABAA R. The labeling was absent from a lysate prepared from knockout animals.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-A R delta
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. Receptors in this group operate as GABA-gated Cl- channels. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Typical GABAA receptors have some combination of an alpha, beta, and gamma subunit. In select neurons, however, a δ subunit replaces the gamma -subunit. It would appear that δ subunits have a preference for various alpha -beta combinations. In cerebellar granule cells, the δ subunit contributes to a unique alpha 6 beta δ heteromer. δ subunit receptors may function to limit the spread of excitatory impulses to dendritically-complexed neurons. They also appear to be sensitive to steroid modulation. The rat δ subunit is a 50 kDa, 433 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 4 - 246). The rat and mouse N-terminal extracellular domains (ECD) (aa 17 - 248) are 99% aa identical; the mouse and human ECD are 95% aa identical.
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gamma-Aminobutyric Acid Type A Receptor, delta Polypeptide
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