Detection of GABAA R δ N-Terminus by Western Blot. Western blot of mouse cerebellar lysates from wild type (Control) and δ knockout (KO) animals showing specific immunolabeling of the ~52 kDa δ subunit of the GABAA R. The labeling was absent from a lysate prepared from knockout animals.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-A R delta
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. Receptors in this group operate as GABA-gated Cl- channels. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Typical GABAA receptors have some combination of an alpha, beta, and gamma subunit. In select neurons, however, a δ subunit replaces the gamma -subunit. It would appear that δ subunits have a preference for various alpha -beta combinations. In cerebellar granule cells, the δ subunit contributes to a unique alpha 6 beta δ heteromer. δ subunit receptors may function to limit the spread of excitatory impulses to dendritically-complexed neurons. They also appear to be sensitive to steroid modulation. The rat δ subunit is a 50 kDa, 433 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 4 - 246). The rat and mouse N-terminal extracellular domains (ECD) (aa 17 - 248) are 99% aa identical; the mouse and human ECD are 95% aa identical.
Whiting, P.J. et al. (1999) Ann. N.Y. Acad. Sci. 868:645.
Rudolph, U. et al. (2001) Trends Pharmacol. Sci. 22:188.
Shivers, B.D. et al. (1989) Neuron 3:327.
Sommer, B. et al. (1990) DNA Cell Biol. 9:561.
Bianchi, M.T. and R.L. MacDonald (2003) J. Neurosci. 23:10934.
gamma-Aminobutyric Acid Type A Receptor, delta Polypeptide