GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-09047

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, X-Ray Crystallography

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 1F4

Format

Azide and BSA Free
View all GABA-A R alpha 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against alpha 1 beta 2 gamma 2 GABA-A receptor.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free

GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free [NBP3-09047] -

Immunocytochemistry /Immunofluorescence: GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free [NBP3-09047] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix(TM) coverslips stained with NBP3-09047 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-09047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

X-Ray Crystallography

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

FSEC: High affinity and specificity of the 1F4 antibody to alpha 1 beta 2 gamma 2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GABA-A R alpha 1

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide

Alternate Names

EJM, GABAAR alpha 1, GABAARa1, GABRA1

Gene Symbol

GABRA1

Additional GABA-A R alpha 1 Products

Product Documents for GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
GABA Receptors

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