GABA-AR alpha 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87499

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GABA A R alpha 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSHCYMTSLGILFLINILPGTTGQGESRRQEPGDFVKQDIGGLSPKHAPDIPDDSTDNITIFTRILDRLLDGYDNRLRPGLGDAVTEVKTDIYVTSFGPVSDTDMEYTIDVFFRQTWHDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-AR alpha 3 Antibody - BSA Free

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499]

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499]

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499]

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499]

Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in human cell line U-87 MG.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse ventral pallidum shows strong labeling of synaptic fields.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse olfactory bulb shows labelling of both neurons and synapses.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse insular cortex shows strong immunoreactivity in synapses.
Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse thalamus shows strong positivity in the paraventricular thalamic nucleus.
Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human cerebral cortex shows moderate positivity in neuronal processes in neuropil.
Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499]

Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Applications for GABA-AR alpha 3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GABA A R alpha 3

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; P ltl et al., 2003).

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-3 Polypeptide

Alternate Names

GABAARa3, GABRA3

Entrez Gene IDs

2556 (Human)

Gene Symbol

GABRA3

UniProt

P34903 (Human)

Additional GABA A R alpha 3 Products

Product Documents for GABA-AR alpha 3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GABA-AR alpha 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GABA Receptors

Citations for GABA-AR alpha 3 Antibody - BSA Free

Customer Reviews for GABA-AR alpha 3 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review GABA-AR alpha 3 Antibody - BSA Free and earn rewards!

Have you used GABA-AR alpha 3 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies