GABARAP Antibody (5T6W5)

Novus Biologicals | Catalog # NBP3-16376

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5T6W5 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABARAP (O95166). MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GABARAP Antibody (5T6W5)

Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376]

Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376]

Western Blot: GABARAP Antibody (5T6W5) [NBP3-16376] - Western blot analysis of extracts of various cell lines, using GABARAP Rabbit mAb (NBP3-16376) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for GABARAP Antibody (5T6W5)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABARAP

FUNCTION: May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. SUBUNIT: Interacts with GABRG2, TUBA1, ULK1 and NSF. Interacts with beta-tubulin and GPHN. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoskeleton. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. SIMILARITY: Belongs to the MAP1 LC3 family.

Long Name

gamma-Aminobutyric Acid Type A Receptor Associated Protein

Alternate Names

Apg8p1, ATG8A, FLC3B, MM46

Gene Symbol

GABARAP

Additional GABARAP Products

Product Documents for GABARAP Antibody (5T6W5)

Certificate of Analysis

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Product Specific Notices for GABARAP Antibody (5T6W5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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