Gabpb2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81132

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Gabpb2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DKSAFDIALEKNNAEILVILQEAMQNQVNVNPERANPVTDPVSMAAPFIFTSGEVVNLASLISSTNTKTTSGDPHASTVQFSNSTTS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gabpb2 Antibody - BSA Free

Western Blot: Gabpb2 Antibody [NBP1-81132]

Western Blot: Gabpb2 Antibody [NBP1-81132]

Western Blot: Gabpb2 Antibody [NBP1-81132] - Analysis in control (vector only transfected HEK293T lysate) and GABPB2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Gabpb2 Antibody [NBP1-81132]

Immunocytochemistry/ Immunofluorescence: Gabpb2 Antibody [NBP1-81132]

Immunocytochemistry/Immunofluorescence: Gabpb2 Antibody [NBP1-81132] - Staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Gabpb2 Antibody [NBP1-81132]

Immunohistochemistry-Paraffin: Gabpb2 Antibody [NBP1-81132]

Immunohistochemistry-Paraffin: Gabpb2 Antibody [NBP1-81132] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Applications for Gabpb2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gabpb2

Gabpb2 encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

GA binding protein transcription factor, beta subunit 2, GA-binding protein subunit beta-2, GABP subunit beta-2, GABPB-2, MGC29891

Entrez Gene IDs

126626 (Human)

Gene Symbol

GABPB2

UniProt

Q8TAK5 (Human)

Additional Gabpb2 Products

Product Documents for Gabpb2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Gabpb2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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