Recombinant Human GAD1/GAD67 His Protein

Name: Recombinant Human GAD1/GAD67 His Protein
Brand: Novus Biologicals
SKU: NBP2-51655
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-51655

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all GAD1/GAD67 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-224 of Human GAD1/GAD67

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS TANTNMPSDM RECWLLR

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GAD1/GAD67 His Protein

SDS-PAGE: Recombinant Human GAD1/GAD67 His Protein [NBP2-51655]

SDS-Page: Recombinant Human GAD1/GAD67 Protein [NBP2-51655] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-51655

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for Recombinant Human GAD1/GAD67 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human GAD1/GAD67 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.