GADD153/CHOP Antibody (JM10-31)

Novus Biologicals | Catalog # NBP2-66856

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM10-31 expressed in HEK293
View all GADD153 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human GADD153/CHOP aa 135-169 / 169. (SwissProt: P35638 Human; SwissProt: P35639 Mouse; SwissProt: Q62857 Rat)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GADD153/CHOP Antibody (JM10-31)

Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
GADD153/CHOP Antibody (JM10-31) Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Western blot analysis on different lysates with NBP2-66856 at 1/5,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66856 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
GADD153/CHOP Antibody (JM10-31) Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856]

Western blot analysis on different lysates with NBP2-66856 at 1/5,000 dilution. Lane 1: C2C12 cell lysate Lane 2: C2C12 treatment with 300 nM thapsigargin for 18h cell lysate Lane 3: C6 cell lysate Lane 4: C6 treatment with 300 nM thapsigargin for 18h cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-66856, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 19 kDa Observed band size: 27 kDa

Applications for GADD153/CHOP Antibody (JM10-31)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GADD153

GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Long Name

Growth Arrest and DNA Damage-inducible Protein GADD153

Alternate Names

CEBP zeta, CHOP, CHOP10, DDIT3

Gene Symbol

DDIT3

Additional GADD153 Products

Product Documents for GADD153/CHOP Antibody (JM10-31)

Certificate of Analysis

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Product Specific Notices for GADD153/CHOP Antibody (JM10-31)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GADD153/CHOP Antibody (JM10-31)

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Protocols

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