GADD153/CHOP Antibody (S02-6A3)

Novus Biologicals | Catalog # NBP3-14934

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-6A3 expressed in HEK293
View all GADD153 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human GADD153/CHOP (Uniprot#: P35638)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GADD153/CHOP Antibody (S02-6A3)

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934] - Western blot detection of GADD153/CHOP in K562, CHO-K1, Hela cell lysates using GADD153/CHOP Rabbit mAb (1:1000 diluted). Predicted band size: 19KDa. Observed band size: 27KDa.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934] - Immunohistochemistry of GADD153/CHOP in paraffin-embedded Human breast cancer tissue using GADD153/CHOP Rabbit mAb at dilution 1/1

Applications for GADD153/CHOP Antibody (S02-6A3)

Application
Recommended Usage

Immunohistochemistry

1/20-1/50

Immunohistochemistry-Paraffin

1/20-1/50

Western Blot

1/1000-1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GADD153

GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Long Name

Growth Arrest and DNA Damage-inducible Protein GADD153

Alternate Names

CEBP zeta, CHOP, CHOP10, DDIT3

Gene Symbol

DDIT3

Additional GADD153 Products

Product Documents for GADD153/CHOP Antibody (S02-6A3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GADD153/CHOP Antibody (S02-6A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GADD153/CHOP Antibody (S02-6A3)

Customer Reviews for GADD153/CHOP Antibody (S02-6A3)

There are currently no reviews for this product. Be the first to review GADD153/CHOP Antibody (S02-6A3) and earn rewards!

Have you used GADD153/CHOP Antibody (S02-6A3)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies