GADD34 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-89800
![GADD34 Antibody - BSA Free Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]](https://resources.rndsystems.com/images/products/nbp1-89800_rabbit-polyclonal-gadd34-antibody-15520258564372.jpg "Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QPGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCP
Reactivity Notes
Use in Mouse reported in scientific publication (PMID: 32435966).
Localization
Endoplasmic reticulum
Marker
ER Stress Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GADD34 Antibody - BSA Free
Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]
Staining of human prostate shows strong cytoplasmic granular positivity in glandular cells.![GADD34 Antibody - BSA Free Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]](https://resources.rndsystems.com/images/products/nbp1-89800_rabbit-polyclonal-gadd34-antibody-15520258303349.jpg "Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]")
Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]
Staining of human rectum shows strong cytoplasmic granular positivity in glandular cells.![GADD34 Antibody - BSA Free Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]](https://resources.rndsystems.com/images/products/nbp1-89800_rabbit-polyclonal-gadd34-antibody-15520258515122.jpg "Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]")
Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]
Staining of human pancreas shows strong cytoplasmic granular positivity in exocrine glandular cells.![GADD34 Antibody - BSA Free Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]](https://resources.rndsystems.com/images/products/nbp1-89800_rabbit-polyclonal-gadd34-antibody-1552025823120.jpg "Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]")
Immunohistochemistry-Paraffin: GADD34 Antibody - BSA Free [NBP1-89800]
Staining of human endometrium shows strong cytoplasmic granular positivity in glandular cells.Applications for GADD34 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
IHC-P, Retrieval method: HIER pH6
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GADD34
Long Name
Protein phosphatase 1 regulatory subunit 15A
Alternate Names
PPP1R15A
Entrez Gene IDs
23645 (Human)
Gene Symbol
PPP1R15A
UniProt
Additional GADD34 Products
Product Documents for GADD34 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GADD34 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for GADD34 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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