Galectin-1 Antibody (RM0081-9J26) - Azide and BSA Free

A: The protocol for tissue samples preparation for the WB with Galectin 1 antibody (# NBP1-95445 ) is as follows: 1. Wash the tissue with cold PBS. 2. Chop the tissue into small pieces, tip tissue sample into liquid nitrogen. 3. Start crushing tissue sample with pestle. Crushing sample to a powder before the liquid nitrogen completely evaporates. 4. Add appropriate volume of 1X hot lysis buffer, 100C, 5min. 5. Sonicate the tissue for 10-15seconds. 6. Spin tissue debris down at 10000rpm, 10min. 7. Carefully remove the lipid on the surface of the supernatant. Save supernatant as whole tissue lysate, and discard the pellet. 8. Determine the protein concentration by BCA protein assay. 9. Adjust concentration to 2 mg/ml with Lysis buffer. 10. Add an equal volume of 2X Laemmli Sample Buffer (final concentration of 1 mg/ml). 11. Boil each cell lysate in sample buffer at 100 C for 5 min and aliquot.