GALNT6 Antibody (4C10) - Azide and BSA Free

Novus Biologicals | Catalog # H00011226-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C10

Format

Azide and BSA Free
View all GALNT6 Primary Antibodies »

Product Specifications

Immunogen

GALNT6 (NP_009141, 523 a.a. ~ 622 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IMYSCHGLGGNQYFEYTTQRDLRHNIAKQLCLHVSKGALGLGSCHFTGKNSQVPKDEEWELAQDQLIRNSGSGTCLTSQDKKPAMAPCNPSDPHQLWLFV

Reactivity Notes

Human. Other species not tested.

Specificity

GALNT6 - UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for GALNT6 Antibody (4C10) - Azide and BSA Free

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01]

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01]

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01] - GALNT6 monoclonal antibody (M01), clone 4C10 Analysis of GALNT6 expression in A-431.
Western Blot: GALNT6 Antibody (4C10) [H00011226-M01]

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01]

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01] - Analysis of GALNT6 over-expressed 293 cell line, cotransfected with GALNT6 Validated Chimera RNAi ( Cat # H00011226-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GALNT6 monoclonal antibody (M01), clone 4C10 (Cat # H00011226-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: GALNT6 Antibody (4C10) [H00011226-M01]

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01]

Western Blot: GALNT6 Antibody (4C10) [H00011226-M01] - Analysis of GALNT6 expression in transfected 293T cell line by GALNT6 monoclonal antibody (M01), clone 4C10.Lane 1: GALNT6 transfected lysate(71 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: GALNT6 Antibody (4C10) [H00011226-M01] - Detection limit for recombinant GST tagged GALNT6 is approximately 0.1ng/ml as a capture antibody.

Applications for GALNT6 Antibody (4C10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for RNAi vaidation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GALNT6

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin.

Alternate Names

EC 2.4.1.41, GalNAc transferase 6, GalNAcT6, GALNAC-T6, Polypeptide GalNAc transferase 6, polypeptide N-acetylgalactosaminyltransferase 6, pp-GaNTase 6, Protein-UDP acetylgalactosaminyltransferase 6, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6, UDP-N-acetyl-alpha-D-galactosamine: polypeptideN-acetylgalactosaminyltransferase 6, UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 6 (GalNAc-T6)

Entrez Gene IDs

11226 (Human)

Gene Symbol

GALNT6

OMIM

605148 (Human)

UniProt

NP_009141 (Human)

Additional GALNT6 Products

Product Documents for GALNT6 Antibody (4C10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GALNT6 Antibody (4C10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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