gamma Tubulin Antibody [Cy3]

Novus Biologicals | Catalog # NB120-11319

Novus Biologicals

Key Product Details

Species Reactivity

Human, Canine, Chicken

Applications

Immunocytochemistry/ Immunofluorescence

Label

Cy3 (Excitation = 553 nm, Emission = 569 nm)

Antibody Source

Polyclonal Rabbit IgG
View all gamma Tubulin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: EEFATEGTDRKDVFFY-K, corresponding to amino acids 38-53 of Human gamma Tubulin.

Reactivity Notes

The antigen sequence is identical in mouse and rat Gamma-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast Gamma-tubulin).

Localization

Centrosomal

Marker

Centrosome Marker

Specificity

Does not react with alpha, beta, omega and eta Tubulin. The IF testing of this product is performed using chicken fibroblasts. We have not performed IF testing using human cells. This antibody is tested in western blotting using the following cell lysates ; U87, HeLa, Jurkat, C2C12, Rat2, MDCK, and A431.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gamma Tubulin Antibody [Cy3]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [Cy3] [NB120-11319] - HeLa cells were fixed and permeabilized with methanol followed by aceton. Fixed cells were stained directly with 1:200 Rabbit Anti-gamma Tubulin-Cy3

Applications for gamma Tubulin Antibody [Cy3]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
IF: Use at a dilution of 1/200. This concentration was determined by staining of methanol/acetonefixed chicken fibroblast cells.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10 mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma Tubulin

Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for gamma Tubulin Antibody [Cy3]

Certificate of Analysis

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Product Specific Notices for gamma Tubulin Antibody [Cy3]

Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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