gamma Tubulin Antibody

Novus Biologicals | Catalog # NB120-11318

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Canine, Chicken

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all gamma Tubulin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: EEFATEGTDRKDVFFY-K, corresponding to amino acids 38-53 of Human gamma Tubulin.

Reactivity Notes

The antigen sequence is identical in mouse and rat Gamma-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast Gamma-tubulin).

Localization

Centrosomal

Marker

Centrosome Marker

Specificity

Does not react with alpha, beta, omega and eta Tubulin. The IF testing of this product is performed using chicken fibroblasts. We have not performed IF testing using human cells. This antibody is tested in western blotting using the following cell lysates ; U87, HeLa, Jurkat, C2C12, Rat2, MDCK, and A431.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gamma Tubulin Antibody

Western Blot: gamma Tubulin Antibody [NB120-11318]

Western Blot: gamma Tubulin Antibody [NB120-11318]

Western Blot: gamma Tubulin Antibody [NB120-11318] - Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 ug/mL Anti-gamma-Tubulin, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody [NB120-11318]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody [NB120-11318]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in Rat2 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody [NB120-11318]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody [NB120-11318]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody [NB120-11318]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody [NB120-11318]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody [NB120-11318]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody [NB120-11318]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in HeLa cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.

Applications for gamma Tubulin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.09% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma Tubulin

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for gamma Tubulin Antibody

Certificate of Analysis

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Product Specific Notices for gamma Tubulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for gamma Tubulin Antibody

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Protocols

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FAQs for gamma Tubulin Antibody

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  • Q: Does this antibody require methanol fixation for immunofluorescence use?

    A: This antibody was tested using methanol/acetone-fixed chicken fibroblast cells but that does not necessarily mean that methanol fixation is required. This antibody should work using whatever protocol you prefer.

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