Gankyrin Antibody (OTI3F6)

Novus Biologicals | Catalog # NBP2-02199

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F6
View all Gankyrin Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PSMD10 (NP_002805) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gankyrin Antibody (OTI3F6)

Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.
Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Ovary tissue using anti-PSMD10 mouse monoclonal antibody.
Flow Cytometry: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Flow Cytometry: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Flow Cytometry: PSMD10 Antibody (3F6) [NBP2-02199] - Analysis of Jurkat cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Western Blot: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD10.
Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded endometrium tissue using anti-PSMD10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Kidney tissue using anti-PSMD10mouse monoclonal antibody.
Flow Cytometry: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Flow Cytometry: Gankyrin Antibody (OTI3F6) [NBP2-02199]

Flow Cytometry: PSMD10 Antibody (3F6) [NBP2-02199] - Analysis of Hela cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Applications for Gankyrin Antibody (OTI3F6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gankyrin

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.

Alternate Names

26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, ankyrin repeat protein, dJ889N15.2, Gankyrin, hepatocellular carcinoma-associated protein p28-II, p28, p28(GANK), proteasome (prosome, macropain) 26S subunit, non-ATPase, 10

Entrez Gene IDs

5716 (Human)

Gene Symbol

PSMD10

Additional Gankyrin Products

Product Documents for Gankyrin Antibody (OTI3F6)

Certificate of Analysis

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Product Specific Notices for Gankyrin Antibody (OTI3F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Gankyrin Antibody (OTI3F6)

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Protocols

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