GAPDH-2 Antibody (Hs-8) - BSA Free

Novus Biologicals | Catalog # NB500-456

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # Hs-8

Format

BSA Free
View all GAPDH-2 Primary Antibodies »

Product Specifications

Immunogen

This GAPDH-2 antibody was developed against the full length human protein extracted from sperms.

Specificity

The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein".

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for GAPDH-2 Antibody (Hs-8) - BSA Free

Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456]

Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456]

Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.
GAPDH-2 Antibody (Hs-8) Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456]

Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456]

Separation of human GAPDHS positive cells (red-filled) from GAPDHS negative cells (black-dashed) in Analysis (intracellular staining) of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 ug/ml) GAM APC.

Applications for GAPDH-2 Antibody (Hs-8) - BSA Free

Application
Recommended Usage

Flow Cytometry

3-12 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

1:1000
Application Notes
Western Blot Detects bands of approximately 220, 50, 34 and 21 kDa. Immunocytochemistry: Staining technique: Membrane permeabilization (acetone) is essential.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

Tris buffered saline (TBS), pH 8.0

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GAPDH-2

This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining 7 transcripts combined account for 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase 2

Alternate Names

GAPD2, GAPDH2, GAPDHS, HSD-35

Entrez Gene IDs

56 (Human)

Gene Symbol

GAPDHS

Additional GAPDH-2 Products

Product Documents for GAPDH-2 Antibody (Hs-8) - BSA Free

Certificate of Analysis

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Product Specific Notices for GAPDH-2 Antibody (Hs-8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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