GAPDH-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03522

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GAPDH-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 179-408 of human GAPDH-2 (NP_055179.1). ASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAPDH-2 Antibody - BSA Free

Western Blot: GAPDH-2 AntibodyBSA Free [NBP3-03522]

Western Blot: GAPDH-2 AntibodyBSA Free [NBP3-03522]

Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
GAPDH-2 Antibody - BSA Free

Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] -

Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Rat heart using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol
GAPDH-2 Antibody - BSA Free

Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] -

Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded human breast using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol
GAPDH-2 Antibody - BSA Free

Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] -

Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol

Applications for GAPDH-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAPDH-2

GAPDHS encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase 2

Alternate Names

GAPD2, GAPDH2, GAPDHS, HSD-35

Gene Symbol

GAPDHS

Additional GAPDH-2 Products

Product Documents for GAPDH-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GAPDH-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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