GAPDH Antibody

Novus Biologicals | Catalog # NB300-327

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bacteria, Bovine, Chicken, Equine, Fungi, Invertebrate, Yeast

Cited:

Human, Mouse, Rat, Avian - Chicken, Bacteria, Bovine, Fungi, Invertebrate, Yeast

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all GAPDH Primary Antibodies »

Product Specifications

Immunogen

This GAPDH antibody was developed against full length recombinant human GAPDH

Reactivity Notes

Bacteria reactivity reported in scientific literature (PMID: 31413153). Fungi reactivity reported in scientific literature (PMID:31413153).

Marker

Cytosolic Marker

Clonality

Polyclonal

Host

Rabbit

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GAPDH Antibody (NB300-327) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-GAPDH Antibody: Cited in 49 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-327]

Western Blot: GAPDH Antibody [NB300-327]

GAPDH-Antibody-Western-Blot-NB300-327-img0009.jpg
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-327] - Analysis of HeLa cells stained with rabbit pAb to GAPDH, dilution 1:2000 in red. Blue is Hoescht staining of nuclear DNA. The GAPDH antibody produces diffuse cytoplasmic staining of cells.
Western Blot: GAPDH Antibody [NB300-327]

Western Blot: GAPDH Antibody [NB300-327]

Western Blot: GAPDH Antibody [NB300-327] - Theoretical molecular weight: 36 kDa. Detection of GAPDH in mouse liver.
Western Blot: GAPDH Antibody [NB300-327]

Western Blot: GAPDH Antibody [NB300-327]

Western Blot: GAPDH Antibody [NB300-327] - Western blot analysis of extracts from Jurkat and HeLa cells using NB300-327 at 1:1000. Theoretical molecular weight: 36 kDa.
Western Blot: GAPDH Antibody [NB300-327]

Western Blot: GAPDH Antibody [NB300-327]

Western Blot: GAPDH Antibody [NB300-327] - Analysis of different cell cytosolic or nuclear enriched fractions, GAPDH antibody, dilution 1:20,000 (Red): [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with mouse mAb to the nuclear RNA binding protein SF3B4, dilution 1:1,000 (Green). In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-327] - The GAPDH antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-327] - Confocal immunofluorescence analysis of HeLa cells using GAPDH (NB300-327) antibody (green). Nuclei was counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-327] - IF Confocal analysis of C2C12 cells using GAPDH antibody (NB300-327, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Simple Western: GAPDH Antibody [NB300-327]

Simple Western: GAPDH Antibody [NB300-327]

Simple Western: GAPDH Antibody [NB300-327] - Simple Western lane view shows a specific band for GAPDH in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higer than predicted 36 kDa molecular weight.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-327] -

nb300-327_rabbit-polyclonal-gapdh-antibody-25520231539521.jpg
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-327] -

Representative immunoblot of KDR and FGFR CAM protein expression levels examined by Western blotting. GNS, graphene nanosheet; NG, graphite nanoparticle; ND, diamond nanoparticle; C60, fullerene C60; MWNT, multi-wall nanotube; KDR, vascular endothelial growth factor receptor; FGFR, fibroblast growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-327] -

Western Blot: GAPDH Antibody [NB300-327] - Increased PIP expression in the lungs of PF‐PH compared to PF ratsA, BRelative expression of PIP mRNA (A) & protein (B) normalized to GAPDH.CRepresentative images & PIP quantification.Data information: Values are expressed as mean ± SEM. The number of samples per group for each experiment is included within each bar graph (t‐test; *P < 0.05, **P < 0.01). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31468711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-327] -

Western Blot: GAPDH Antibody [NB300-327] - Effects of subchronic administration (7 & 14 days) of therapeutic relevant concentration of Brex (Brex: 300 nM) & interaction between Brex & 5-HT1A receptor (5-HT1AR) antagonist WAY100635 (WAY: 10 μM) on protein expression of 5-HT1A (panel A) & 5-HT7 (panel B) receptor in the plasma membrane fraction of cortical primary cultured astrocytes. In left side histograms, ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT1AR & 5-HT7R per GAPDH. * p < 0.05, ** p < 0.01: relative to control (Brex-free) by one-way analysis of variance (ANOVA) with Tukey’s post-hoc test, & @ p < 0.05: relative to Brex for 14 days by Student’s T-test. Right side panels indicate their pseudo-gel images using capillary immunoblotting. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35743014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-327] -

Western Blot: GAPDH Antibody [NB300-327] - Online lung microarray analysis revealed PIP as one of the Slug transcriptional targets mediating vascular cell proliferation in PF‐PHAHeatmap of the 20 genes upregulated in GSE24988 microarray & known to be Slug transcriptional target.BVenn diagram showing the overlap between Slug targets implicated in cell proliferation & known to be extracellular.CHeatmap of the seven transcriptional targets of Slug known to promote proliferation & to be extracellular.D, ERelative expression of prolactin‐induced protein (PIP) mRNA (D) & protein (E) expression normalized to GAPDH in PF & PF‐PH patients.FRepresentative images of PIP immunohistochemistry showing its extracellular localization & quantification.G–JProliferation assay on healthy fibroblast (G), pulmonary arterial EC (H), & healthy pulmonary arterial SMC (I & J) in the presence or absence of PIP.Data information: Values are expressed as mean ± SEM. The number of samples per group for each experiment is included within each bar graph. Statistical test: panels (D–F): t‐test; panels (G–J): ANOVA (*P < 0.05, **P < 0.01 ***P < 0.001). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31468711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-327] -

Western Blot: GAPDH Antibody [NB300-327] - Growth of lactobacilli on IECs & their influence towards C. albicans cytotoxicity, adhesion, hyphal length & translocation. (A) LDH release of IECs colonized (Pre-Inc.) or simultaneously colonized (w/o Pre-Inc.) with L. rhamnosus (L.r.) at different MOI (5, 50 or 250) & infected or not with C. albicans (C.a.) (MOI 1) & measured at 24 h post-infection. (B) Growth of L. paracasei (L.p.), L. rhamnosus, L. salivarius (L.s.), L. fermentum (L.f.), & L. brevis (L.b.) on IECs. (C) Percentage of C. albicans adhered to IECs colonized with different Lactobacillus species (MOI 50) at 1 h post-infection. (D) C. albicans hyphal induction on IECs or on plastic colonized with L. rhamnosus or L. brevis (MOI 50) at 4 h post-infection. Results were normalized to C. albicans single infection. (E) Translocation of C. albicans (MOI 1) across IECs colonized with L. rhamnosus or L. brevis (MOI 50) at 24 h post-infection. (F) Assessment of epithelial barrier integrity measured as the loss of transepithelial electrical resistance (TEER) in response to L. rhamnosus or L. brevis (MOI 50) colonization & C. albicans infection in the presence or absence of Lactobacillus colonization at 24 h post-infection. Data are TEER loss in percentage of uninfected host cells (before pre-incubation). (G) E-Cadherin protein expression analyzed by western blot compared to GAPDH in IECs that were left uninfected or colonized with L. rhamnosus (MOI 50) & infected with C. albicans for 6, 12 & 24 h. Data are mean±s.e.m. *P<0.05, **P<0.01, ***P<0.005 (t-test). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31413153), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody Simple Western: GAPDH Antibody [NB300-327] -

Simple Western: GAPDH Antibody [NB300-327] -

Panel (D) indicates the expression of connexin43 (Cx43) and pannexin1 (PANX1) in the plasma membrane fraction of the PPN between wild type (grey columns) and S286L-TG (black columns). Ordinates indicate the mean SD (n = 6) of the relative expression of connexin43 and pannexin1 per GAPDH. Right-side panels indicate the pseudo-gel images of connexin43, pannexin1 and GAPDH, obtained using capillary immunoblotting. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/40564986/), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GAPDH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:10000

Simple Western

1:500

Western Blot

1:5000
Application Notes

This GAPDH antibody is useful Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at approx. 36kDa, and on cells in tissue culture the antibody stains in a punctate cytoplasmic fashion.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:500, apparent MW was 43 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 3 reviews rated 5 using NB300-327 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Supplied as serum

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAPDH

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References

1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase

Alternate Names

G3PDH

Entrez Gene IDs

2597 (Human); 14433 (Mouse); 24383 (Rat); 786101 (Bovine)

Gene Symbol

GAPDH

UniProt

P10096 (Bovine)

Additional GAPDH Products

Product Documents for GAPDH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GAPDH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GAPDH Antibody (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell lysate from HeLa cells
    Species: Human
    Verified Customer | Posted 09/25/2014
  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 06/13/2014
    IF Confocal analysis of C2C12 cells using GAPDH antibody (NB300-327, 1:20).
    GAPDH Antibody NB300-327
  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 05/21/2014
    Staining of endogenous GAPDH in HeLa at 1:50
    GAPDH Antibody NB300-327

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GAPDH Antibody

Showing  1 - 5 of 8 FAQs Showing All

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

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