Gasdermin D Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93428

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Gasdermin D Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human Gasdermin D (NP_079012.3). DGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gasdermin D Antibody - BSA Free

Western Blot: GSDMDC1 AntibodyAzide and BSA Free [NBP2-93428]

Western Blot: GSDMDC1 AntibodyAzide and BSA Free [NBP2-93428]

Western Blot: GSDMDC1 Antibody [NBP2-93428] - Western blot analysis of extracts of various cell lines, using GSDMDC1 antibody (NBP2-93428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
GSDMDC1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] -

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of PC-12 cells using GSDMD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
GSDMDC1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] -

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of HeLa cells using GSDMD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
GSDMDC1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] -

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of PC-12 cells using GSDMD Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
GSDMDC1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] -

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of A-431 cells using GSDMD (Full Length+N terminal) Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
GSDMDC1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] -

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of U2OS cells using GSDMD Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
GSDMDC1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] -

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of PC-3 cells using GSDMD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
GSDMDC1 Antibody - Azide and BSA Free

Western Blot: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] -

Western Blot: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Western blot analysis of recombinant GSDMD-FL and N+C Protein using GSDMD Rabbit pAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
GSDMDC1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] -

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of NIH/3T3 cells using GSDMD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Gasdermin D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gasdermin D

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Alternate Names

DF5L, DFNA5L, gasdermin domain containing 1, Gasdermin domain-containing protein 1, gasdermin-D, GSDMDC1

Gene Symbol

GSDMD

Additional Gasdermin D Products

Product Documents for Gasdermin D Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Gasdermin D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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