Gastrin-releasing Peptide R/GRPR Antibody - BSA Free

Novus Biologicals | Catalog # NLS830

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bat, Canine, Equine, Monkey, Primate, Rabbit

Cited:

Human

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Gastrin-releasing Peptide R/GRPR Primary Antibodies »

Product Specifications

Immunogen

Synthetic 18 amino acid peptide from 2nd extracellular domain of human Gastrin-releasing Peptide R/GRPR.

Epitope

2nd extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Mouse (83%), Rat (83%), Hamster (83%).

Specificity

Human GRPR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Gastrin-releasing Peptide R/GRPR Antibody - BSA Free

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody [NLS830] - Staining in stomach tissue.
Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody [NLS830] - Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NLS830] - Analysis of anti-GRPR antibody with human pancreas, islet of Langerhans at 4 ug/ml.
Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody [NLS830] - Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NLS830] - Analysis of anti-GRPR antibody with human lung, non-small cell carcinoma.
Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody [NLS830] - Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NLS830] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody - BSA Free [NLS830]

Immunohistochemistry-Paraffin: Gastrin-releasing Peptide R/GRPR Antibody [NLS830] - Brain Cerebellum Purkinje Neurons

Applications for Gastrin-releasing Peptide R/GRPR Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

4 ug/ml

Western Blot

1:1000 - 1:10000
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Gastrin-releasing Peptide R/GRPR

Gastrin-releasing peptide receptor (GRPR) is a Bombesin Receptor. In addition to its involvement in autism and mental retardation, GRPR may also play a role in the progression of cancer. Nicotine exposure induces an increase in GRPR expression in the lung Shriver et al. (2000). GRPR has been reported in brain, breast, colon, lung, lymph node, ovary, placenta, prostate, stomach, and uterus. It has also been shown to be expressed in several cancers, including those in breast, colon, lung, ovary, pancreas, and prostate.

Long Name

Gastrin-releasing Peptide Receptor

Alternate Names

Gastrin-releasing PeptideR, Gastrinreleasing PeptideR, GRPR

Entrez Gene IDs

NM_005314 (Human)

Gene Symbol

GRPR

UniProt

P30550 (Human)

Additional Gastrin-releasing Peptide R/GRPR Products

Product Documents for Gastrin-releasing Peptide R/GRPR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Gastrin-releasing Peptide R/GRPR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Gastrin-releasing Peptide R/GRPR Antibody - BSA Free

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Protocols

View specific protocols for Gastrin-releasing Peptide R/GRPR Antibody - BSA Free (NLS830):

Immunohistochemistry Protocol for Neuromedin C Antibody (NLS830):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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