GCC185 Antibody

Novus Biologicals | Catalog # NBP1-20922

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GCC185 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QKLTNKNNKIED corresponding to internal region according to NP_055450.1, NP_852118.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GCC185 Antibody

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - (3.8 ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GCC185 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 60 kDa band in human lymph node and testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185 kDa band according to NP_055450.1 and of 196 kDa band according to NP_852118.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Paraffin embedded Human Spleen, Small Intestine and Kidney.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GCC185

The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Alternate Names

185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC protein, 185-kD, GCC185GRIP and coiled-coil domain-containing 2, Golgi coiled-coil protein GCC185, GRIP and coiled-coil domain containing 2, GRIP and coiled-coil domain-containing protein 2, KIAA0336REN53, Ran-binding protein 2-like 4, RANBP2L4, Renal carcinoma antigen NY-REN-53

Entrez Gene IDs

9648 (Human)

Gene Symbol

GCC2

UniProt

Q8IWJ2 (Human), NP_852118.1 (Human), NP_055450.1 (Human)

Additional GCC185 Products

Product Documents for GCC185 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GCC185 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GCC185 Antibody

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Protocols

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