GCNT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88781

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVPWKYVINTCGQDFPLKTNREIVQHLKGFKGKNITPGVLPPDHAIKRTKYVHQEHTDKGGFFVKNTNILKTSPPHQLTIYFGTAYVALTREFVDFVLRDQRAIDLLQWSKDT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCNT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GCNT2 Antibody [NBP1-88781]

Immunocytochemistry/ Immunofluorescence: GCNT2 Antibody [NBP1-88781]

Immunocytochemistry/Immunofluorescence: GCNT2 Antibody [NBP1-88781] - Immunofluorescent staining of human cell line CACO-2 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781]

Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781]

Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781]

Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781]

Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781] - Staining of human Duodenum shows strong positivity in Golgi apparatus in glandular cells.
Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781]

Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781]

Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781] - Staining of human Kidney shows strong positivity in Golgi apparatus in cells in tubules.
Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781]

Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781]

Immunohistochemistry-Paraffin: GCNT2 Antibody [NBP1-88781] - Staining of human Prostate shows strong positivity in Golgi apparatus in glandular cells.

Applications for GCNT2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glucosaminyl (N-acetyl) Transferase 2/GCNT2

GCNT2 encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Long Name

Glucosaminyl (N-Acetyl) Transferase 2, I-Branching Enzyme (I Blood Group)

Alternate Names

CCAT, CTRCT13, GCNT2C, NACGT1, NAGCT1, ULG3

Gene Symbol

GCNT2

Additional Glucosaminyl (N-acetyl) Transferase 2/GCNT2 Products

Product Documents for GCNT2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GCNT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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FAQs

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