Gelsolin/GSN Antibody (5O10O1)

Novus Biologicals | Catalog # NBP3-16138

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5O10O1 expressed in HEK293
View all Gelsolin/GSN Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Gelsolin/GSN (NP_000168.1). DQTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gelsolin/GSN Antibody (5O10O1)

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138] - Western blot analysis of extracts of various cell lines, using Gelsolin/GSN Rabbit mAb (NBP3-16138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138] - Immunohistochemistry of paraffin-embedded mouse brain using Gelsolin/GSN Rabbit mAb (NBP3-16138) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138] - Western blot analysis of extracts of various cell lines, using Gelsolin/GSN Rabbit mAb (NBP3-16138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Applications for Gelsolin/GSN Antibody (5O10O1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody (5O10O1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Gelsolin/GSN Antibody (5O10O1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Customer Reviews for Gelsolin/GSN Antibody (5O10O1)

There are currently no reviews for this product. Be the first to review Gelsolin/GSN Antibody (5O10O1) and earn rewards!

Have you used Gelsolin/GSN Antibody (5O10O1)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies