GEM Antibody

Novus Biologicals | Catalog # NBP2-26121

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GEM Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QKEPHQYSHRNRH corresponding to internal region according to NP_005252.1.

Specificity

Reported variants represent identical protein: NP_005252.1, NP_859053.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GEM Antibody

Western Blot: GEM Antibody [NBP2-26121]

Western Blot: GEM Antibody [NBP2-26121]

Western Blot: GEM Antibody [NBP2-26121] - HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with NBP2-26121 (1ug/ml). The same lysates probed with anti-HA tag antibody in the lower panel.
Western Blot: GEM Antibody [NBP2-26121]

Western Blot: GEM Antibody [NBP2-26121]

Western Blot: GEM Antibody [NBP2-26121] - (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for GEM Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing human recombinant GEM bands of approx. 35+45 kDa band are observed. These bands are not observed in the transfected HEK293 with empty vector and the antibody does not cross-react to related protein such as Rem, Rem2L or Rad, provided by Prof. R Andres, Univ. Kentucky, USA. A similar banding pattern is observed in human spleen. The calculated molecular size is 33.9 kDa band according to NP_005252.1.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GEM

The protein encoded by the GEM gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the innerface of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction.Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.(provided by RefSeq)

Alternate Names

GTP binding protein overexpressed in skeletal muscle, GTP-binding mitogen-induced T-cell protein, GTP-binding protein GEM, GTP-binding protein overexpressed in skeletal muscle, kinase-inducible Ras-like protein, KIRMGC26294, RAS-like protein KIR

Entrez Gene IDs

2669 (Human)

Gene Symbol

GEM

Additional GEM Products

Product Documents for GEM Antibody

Certificate of Analysis

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Product Specific Notices for GEM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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