Gemin 3 Antibody

Novus Biologicals | Catalog # NB100-1802

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Gemin 3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 675 and 725 of human DEAD (Asp-Glu-Ala-Asp) Box 20 using the numbering given in entry NP_009135.3 (GeneID 11218).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gemin 3 Antibody

Western Blot: Gemin 3 Antibody [NB100-1802]

Western Blot: Gemin 3 Antibody [NB100-1802]

Western Blot: Gemin 3 Antibody [NB100-1802] - Detection of Human DDX20 on HeLa whole cell lystate using NB100-1802. DDX20 was also immunoprecipitated using rabbit anti-DDX20 antibody NB100-1801 and two others.

Applications for Gemin 3 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Gemin 3

The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. A DEAD box putative RNA helicase, named Gemin 3 which is another component of the SMN complex, has been identified. Gemin 3 interacts directly with SMN, as well as with SmB, SmD2 and SmD3. Immunolocalization studies using mAbs to Gemin 3 show that it colocalizes with SMN in gems. Gemin 3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin 3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.

Alternate Names

Component of gems 3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 20, DEAD box protein 20, DEAD box protein DP 103, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD, DKFZp434H052, DP103DEAD-box protein DP103, EC 3.6.4.13, Gemin-3, GEMIN3gemin-3, probable ATP-dependent RNA helicase DDX20, SMN-interacting protein

Entrez Gene IDs

11218 (Human)

Gene Symbol

DDX20

UniProt

NP_009135.3 (Human)

Additional Gemin 3 Products

Product Documents for Gemin 3 Antibody

Certificate of Analysis

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Product Specific Notices for Gemin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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