GFAP Antibody

Novus Biologicals | Catalog # NB300-141

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Chicken, Equine, Guinea Pig, Rabbit

Cited:

Human, Mouse, Rat, Avian - Chicken, Guinea Pig, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all GFAP Primary Antibodies »

Product Specifications

Immunogen

This GFAP Antibody was developed against recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.

Reactivity Notes

Predicted to work with most mammals. Chicken reactivity reported in scientific literature (PMID: 20844134). Rabbit and Guinea Pig reactivity reported in scientific literature (PMID: 4559710).

Marker

Astrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GFAP Antibody (NB300-141) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-GFAP Antibody: Cited in 110 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GFAP Antibody

Immunohistochemistry: GFAP Antibody [NB300-141]

Immunohistochemistry: GFAP Antibody [NB300-141]

Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 uM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141]

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).
Simple Western: GFAP Antibody [NB300-141]

Simple Western: GFAP Antibody [NB300-141]

Simple Western: GFAP Antibody [NB300-141] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141]

GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0014.jpg
Immunohistochemistry-Paraffin: GFAP Antibody [NB300-141]

Immunohistochemistry-Paraffin: GFAP Antibody [NB300-141]

Immunohistochemistry-Paraffin: GFAP Antibody [NB300-141] - Mouse brain section, 20x magnification. Antibody at 1:1000. Detection with Polymer-HRP. IHC image submitted by a verified customer review.
Western Blot: GFAP Antibody [NB300-141]

Western Blot: GFAP Antibody [NB300-141]

Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.
Western Blot: GFAP Antibody [NB300-141]

Western Blot: GFAP Antibody [NB300-141]

Western Blot: GFAP Antibody [NB300-141] - Analysis of GFAP expression in whole rat cerebellum homogenate.
Western Blot: GFAP Antibody [NB300-141]

Western Blot: GFAP Antibody [NB300-141]

Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141]

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141]

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141]

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Cultured Rat hippocampal neurons. ICC/IF image submitted by a verified customer review.
Immunohistochemistry: GFAP Antibody [NB300-141]

Immunohistochemistry: GFAP Antibody [NB300-141]

Immunohistochemistry: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Frozen: GFAP Antibody [NB300-141]

Immunohistochemistry-Frozen: GFAP Antibody [NB300-141]

Immunohistochemistry-Frozen: GFAP Antibody [NB300-141] - Imaging of mouse brain (cortex), 20x magnification. IHC image submitted by a verified customer review.
GFAP Antibody

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] -

EGFP+ neurons are positive for RABV antigen.Brains were collected from Cre reporter mice fifteen days post-infection, cryosectioned, and EGFP+ regions compared to cell-specific labeling, A) NeuN (blue, neuronal nuclei antibody, 20× fluorescence imaging), B) GFAP (blue, astrocyte antibody, 40× confocal imaging), or C) RABV P antigen (purple) and DAPI nuclear stain (blue, 63× confocal imaging). White arrows in (C) indicate regions positive for RABV P.
GFAP Antibody

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] -

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - EGFP+ neurons are positive for RABV antigen.Brains were collected from Cre reporter mice fifteen days post-infection, cryosectioned, & EGFP+ regions compared to cell-specific labeling, A) NeuN (blue, neuronal nuclei antibody, 20× fluorescence imaging), B) GFAP (blue, astrocyte antibody, 40× confocal imaging), or C) RABV P antigen (purple) & DAPI nuclear stain (blue, 63× confocal imaging). White arrows in (C) indicate regions positive for RABV P. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1002971), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GFAP Antibody

Western Blot: GFAP Antibody [NB300-141] -

Western Blot: GFAP Antibody [NB300-141] - Effects of the repeated administration of NVP CXCR2 20 (NVP; 10 μg/5 μl; i.t.; 16 h & 1 h before CCI & then once a day for 7 days) on the protein levels of CXCR2, IBA1, GFAP, CXCL1, CXCL2, & CXCL3 proteins (A–I) in the spinal cord (A–F) & DRG (G–I) on the 7th day after CCI in rats. The data are presented as the mean fold changes relative to the control ± SEM (5–6 samples per group). Intergroup differences were analyzed using ANOVA with Bonferroni's multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 indicate differences vs. naive rats. #p < 0.05, indicate differences between V-treated & NVP-treated rats. CCI, chronic constriction injury; N, naive; V, vehicle; NVP, NVP CXCR2 20. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31616413), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GFAP Antibody

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] -

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Increased astrocyte activation in IL-1 beta -stimulated two-week old offspring is reduced by maternal LB supplementation. Representative images of fluorescence microscopy of claudin-5+ (location of the brain capillaries, red), GFAP+ astrocyte (green), & DAPI (nuclei, blue). Three to five sections per mouse were examined & at least three mice were examined in each group. Stronger than control SPF GFAP staining was observed around the blood vessel (see arrow) after IL-1 beta insult. Maternal supplemented group (LB) with or without postnatal insult had GFAP levels similar to the control group indicating that LB supplementation prevented astrocyte activation around the BBB endothelium. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32424168), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GFAP Antibody

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] -

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Glial cells density is differently modulated during AD progression.a Retinal slices were immunolabeled with anti-GFAP antibody (green) & Hoechst for nuclei visualization (blue) at different ages of 3xTg-AD & non-Tg mice & density of GFAP signal was quantified as shown in b (**p < 0.01 pre vs early; n = 16 fields/four slices for each condition; two-way ANOVA, Holm-Sidak; ##p < 0.01 for comparison with age-matched non-Tg mice, two-way ANOVA, Holm-Sidak). c Representative multiarea image of retinal slice immunolabeled with anti-Iba1 antibody (green) & Hoechst for nuclei visualization (blue); density of Iba1+ cells was quantified as shown in d (**p < 0.01 vs 3xTg-AD pre; ##p < 0.01 vs age-matched non-Tg mice, n = 16 fields/four slices for each condition, two-way ANOVA, Holm-Sidak method for multiple comparison) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29880901), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GFAP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:1000 - 1:5000

Simple Western

1:10,000

Western Blot

1:5000
Application Notes

In WB a band can be seen at 50-55 kDa representing GFAP. A lower band may be seen around 45 kDa representing a proteolytic fragment derived from the GFAP molecule. GFAP antibody validated for IHC-P from a verified customer review. IHC-Fr has been reported in scientific literature (PMID: 28040732).

See Simple Western Antibody Database for Simple Western validation: tested in human brain lysate (0.05 mg/ml); separated by size, antibody dilution of 1:10,000; detects a band at 50 kDa; matrix was 12-230 kDa.

Reviewed Applications

Read 12 reviews rated 4.7 using NB300-141 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Supplied as serum

Preservative

5mM Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFAP

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein, GFAP immunohistochemistry, GFAP mouse, GFAP rabbit, GFAP stain

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

UniProt

P14136, Q28115 (Bovine)

Additional GFAP Products

Product Documents for GFAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GFAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GFAP Antibody (12)

4.7 out of 5
12 Customer Ratings
5 Stars
75%
4 Stars
17%
3 Stars
8%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 12 reviews Showing All
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  • GFAP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: retina
    Species: Bovine
    Verified Customer | Posted 07/28/2020
    good reactivity
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: retina and 30ug
    Species: Goat
    Verified Customer | Posted 07/28/2020
    Very specific bands observed around 42 kDa and use at a concentration of 1:500
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: retina
    Species: Pig
    Verified Customer | Posted 07/28/2020
    good reactivity. use at 1:500.
    use at 1:500
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: retina
    Species: Human
    Verified Customer | Posted 07/28/2020
    good reaction
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: retina
    Species: Mouse
    Verified Customer | Posted 07/28/2020
    very good reaction
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: retina
    Species: Rat
    Verified Customer | Posted 07/28/2020
    good reaction
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: mixed neuronal primary culture
    Species: Human
    Verified Customer | Posted 06/23/2020
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 06/07/2019
    Mouse brain, 20x magnification.
    Proteinasi K treatment; Primary Ab 1:1000. Detection with Polymer-HRP.
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: PFA Fixed Frozen Section of Mouse Brain
    Species: Mouse
    Verified Customer | Posted 06/29/2017
    IHC GFAP NB300-141 on mouse brain (cortex), 20x
    WD [1:K]; Secondary ab RTU Polymer-HRP
    GFAP Antibody NB300-141
  • GFAP Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Primary mouse cortical astrocytes
    Species: Mouse
    Verified Customer | Posted 04/18/2017
  • GFAP Antibody
    Name: Janosch Heller
    Application: Immunocytochemistry
    Sample Tested: Primary rat cortical neurons
    Species: Rat
    Verified Customer | Posted 03/27/2017
    Fixation: 4% paraformaldehyde (TAAB) in PBS, 15 minutes RT Blocking: 10% donkey serum and 1% BSA in PBS with 0.1% saponin, 1 hour RT Permeabilization: 0.1% saponin Primary Antibody: 1:1000 in PBS with 0.1% saponin, overnight, 4°C Wash: three times wash with PBS with 0.1% saponin, 10 min each Secondary Antibody: Invitrogen goat anti-rabbit Alexa647, 1:500 in PBS with 0.1% saponin, 2 hours, RT Wash: two washes with PBS with 0.1% saponin, 10 min each and three washes with PBS, 10 min each Microscope: Burker Vutara 350 The antibody labels intermediate filaments in cultured astrocytes.
    GFAP Antibody NB300-141
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 01/17/2014
    xenografted mouse brain section : astocyte and human nuclei
    GFAP Antibody NB300-141

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Protocols

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FAQs for GFAP Antibody

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    • Q: I would like to use your GFAP-antibody (cat # NB300-141) in a Western Blot experiment. Could you tell me the dilution of this antibody?
      A: The dilution recommended for Western blot using NB300-141 is 1:50,000.

    • A: NB300-141 has been tested and validated in IHC-Frozen and IF; this is covered by our guarantee.
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