GHRHR Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-04431

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GHRHR Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human GHRHR (NP_000814.2). MDRRMWGAHVFCVLSPLPTVLGHMHPECDFITQLREDESACLQAAEEMPNTTLGCPATWDGLLCWPTAGSGEWVTLPCPDFFSHFSSESGAVKRDCTITGWSEPFPPYPVACPVPLELLAEEESYFSTVK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GHRHR Antibody - BSA Free

GHRHR Antibody - BSA Free

Western Blot: GHRHR Antibody - BSA Free [NBP3-04431] -

Western Blot: GHRHR Antibody - BSA Free [NBP3-04431] - Western blot analysis of various lysates, using GHRHR Rabbit pAb (A8421) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
GHRHR Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GHRHR Antibody - BSA Free [NBP3-04431] -

Immunocytochemistry/ Immunofluorescence: GHRHR Antibody - BSA Free [NBP3-04431] - Immunofluorescence analysis of rat pituitary gland cells using GHRHR Rabbit pAb (A8421) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for GHRHR Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GHRHR

GHRHR belongs to the glucagon family and is produced in the hypothalamus as a preproprotein. The preproprotein is cleaved to form a 44 aa factor, also called somatocrinin, that acts to stimulate growth hormone release from the pituitary. Variant receptors for somatocrinin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism.

Long Name

Growth Hormone Releasing Hormone Receptor

Alternate Names

GHRFR, GRFR, IGHD1B, Little

Gene Symbol

GHRHR

Additional GHRHR Products

Product Documents for GHRHR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GHRHR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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