GIT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86144

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GIT1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DLDDQHDYDSVASDEDTDQEPLRSTGATRSNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVSSSLSDELRRLQREIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit GIT1 Antibody - BSA Free (NBP1-86144) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-GIT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GIT1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody [NBP1-86144]

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody [NBP1-86144]

Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-86144] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human kidney shows strong cytoplasmic positivity in glomeruli.
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
GIT1 Antibody - BSA Free Immunohistochemistry-Paraffin: GIT1 Antibody - BSA Free [NBP1-86144]

Immunohistochemistry-Paraffin: GIT1 Antibody - BSA Free [NBP1-86144]

Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.

Applications for GIT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GIT1

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Long Name

G Protein-Coupled Receptor Kinase Interacting ArfGAP 1

Alternate Names

ARF GAP GIT1

Gene Symbol

GIT1

Additional GIT1 Products

Product Documents for GIT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GIT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GIT1 Antibody - BSA Free

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Protocols

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