Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLIPR2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human GAPR1 protein (between amino acids 90-154) [UniProt Q9H4G4]

Localization

Golgi membrane and extracellular exosomes

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLIPR2 Antibody - BSA Free

Western Blot: GLIPR2 AntibodyBSA Free [NBP2-47126]

Western Blot: GLIPR2 AntibodyBSA Free [NBP2-47126]

Western Blot: GLIPR2 Antibody [NBP2-47126] - Total protein from human liver (lane 1) and human spleen (lane 2) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-GAPR1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: GLIPR2 Antibody - BSA Free [NBP2-47126]

Immunohistochemistry-Paraffin: GLIPR2 Antibody - BSA Free [NBP2-47126]

Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and hematoxylin counterstaining. GLIPR2 is a protein which localizes to Golgi membrane as well as extracellular exosomes, and this GLIPR2 antibody generated a very specific cytoplasmic staining with strong signal in extracellular spaces/around cellular membranes.
Immunohistochemistry-Paraffin: GLIPR2 Antibody - BSA Free [NBP2-47126]

Immunohistochemistry-Paraffin: GLIPR2 Antibody - BSA Free [NBP2-47126]

Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and hematoxylin counterstaining. GLIPR2 is a protein which localizes to Golgi membrane as well as extracellular exosomes, and this GLIPR2 antibody generated a very specific cytoplasmic staining with strong signal in extracellular spaces/around cellular membranes.

Applications for GLIPR2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1: 200 - 1:500

Immunohistochemistry-Paraffin

1: 200 - 1:500

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLIPR2

GLIPR2 is closely related to plant pathogenesis-related (PR-1) proteins, which are upregulated in response to pathogen attack. This protein is found within lipid-enriched microdomains on the cytosolic side of the endomembrane system. GLIPR2 is tightly anchored to membranes and absent from the cytosol, although it does not possess a membrane-spanning domain. Recombinant human GLIPR2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

C9orf19Glioma pathogenesis-related protein 2, chromosome 9 open reading frame 19, GAPR1, GAPR-117kD fetal brain protein, GLI pathogenesis-related 2, GliPR 2, Golgi-associated plant pathogenesis-related protein 1, Golgi-associated PR-1 protein

Gene Symbol

GLIPR2

UniProt

Q9H4G4 (Human)

Additional GLIPR2 Products

Product Documents for GLIPR2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLIPR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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