Glucosylceramidase/GBA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32271

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human, Mouse, Fish - Danio rerio (Zebrafish)

Predicted:

Bovine (92%), Chimpanzee (100%), Porcine (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glucosylceramidase/GBA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glucosylceramidase/GBA. The exact sequence is proprietary.

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 25326392). Mouse reactivity reported in scientific literature (PMID: 30927072).

Localization

Lysosome membrane, Peripheral membrane protein, Lumenal side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glucosylceramidase/GBA Antibody - BSA Free

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271]

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271]

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271] - Lung cancer, using GBA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271]

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271]

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Sample(30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500
Glucosylceramidase/GBA Antibody

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] -

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - MCF-7 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glucosylceramidase/GBA Antibody

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] -

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Wild-type (WT) and GBA knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glucosylceramidase/GBA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Glucosylceramidase/GBA Antibody [NBP1-32271] -

Glucosylceramidase/GBA antibody [C1C3] detects Glucosylceramidase/GBA protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Glucosylceramidase/GBA stained by Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Glucosylceramidase/GBA Antibody - BSA Free

Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Glucosylceramidase/GBA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glucosylceramidase/GBA

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein.

Alternate Names

Alglucerase, GBA, GBA1, Imiglucerase

Gene Symbol

GBA1

UniProt

P04062

Additional Glucosylceramidase/GBA Products

Product Documents for Glucosylceramidase/GBA Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glucosylceramidase/GBA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Glucosylceramidase/GBA Antibody - BSA Free

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Protocols

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