Glut3 Antibody - BSA Free

Immunohistochemistry-Paraffin: Glut3 Antibody - BSA Free [NBP1-89762]

Staining of human fallopian tube shows no membranous positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Glut3 Antibody - BSA Free [NBP1-89762]

Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.

Western Blot: Glut3 Antibody - BSA Free [NBP1-89762] -

High glucose-induced premature senescent HRMECs exhibit diminished glycolysis. (A) Gene Set Enrichment Analysis for Glycolysis gene signature. Transcriptome data from bulk RNA sequencing of three biological replicates of HRMECs cultured with 25 mM D-glucose vs. the 5 mM control at 4 weeks of culture. Gene signature from KEGG Glycolysis was used for the gene set enrichment analysis (GSEA). Heatmaps below depict the leading edges for transcripts responsible for major differences. NES: Normalized Enrichment Score. p adj: adjusted p value. (B) Energy phenotype plot from Seahorse XFe96 analyzer depicting assessment of glycolysis as extracellular acidification rate (ECAR) and mitochondrial respiration as oxygen consumption rate (OCR) at baseline and stressed phenotypes. (C) Glycolysis stress assay to characterize glycolysis in HRMECs under different culture conditions. Injections in the assay were glucose at 20 min, oligomycin at 40 min, and 2-DG at 60 min. (D) Statistical comparison of basal glycolysis measures using Seahorse Glycolysis stress assay in three biological replicates. (E) Western blot analysis to compare expression of glycolysis-related proteins. beta -actin was used as the loading control. *p < 0.05, ns: not significant. One-way ANOVA, with Tukey’s post-hoc analysis was used. C, control in blue; LG in green, 25 mM (+L-glucose); HDG in red, 25 mM (+D-glucose). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36091370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.