Human Glut4 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82174

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human GLUT4 in samples. No significant cross-reactivity or interference between Human GLUT4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 6%

Inter-Assay Precision (Precision between assays) CV% 5%

Recovery for Human Glut4 ELISA Kit (Colorimetric)

Recovery

average = 101% for serum; average = 94% for EDTA plasma; average = 101% for cell culture media

Linearity

Human Glut4 ELISA Kit (Colorimetric)

Scientific Data Images for Human Glut4 ELISA Kit (Colorimetric)

Human Glut4 ELISA Kit (Colorimetric)

ELISA: Human Glut4 ELISA Kit (Colorimetric) [NBP2-82174] -

ELISA: Human Glut4 ELISA Kit (Colorimetric) [NBP2-82174] - Standard Curve Reference

Kit Contents for Human Glut4 ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glut4

The stimulation of glucose uptake by insulin in muscle and adipose tissue requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Alternate Names

SLC2A4

Gene Symbol

SLC2A4

Additional Glut4 Products

Product Documents for Human Glut4 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Glut4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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