Glutamate Receptor 4 Antibody (JE36-81)

Western Blot: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384]

Western Blot: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Western blot analysis of Glutamate Receptor 4 on different lysates with Rabbit anti-Glutamate Receptor 4 antibody (NBP3-32384) at 1/1,000 dilution.

Lane 1: Mouse cerebellum tissue lysate (RIPA lysis)
Lane 2: Mouse cerebellum tissue lysate (hot lysis)
Lane 3: Rat cerebellum tissue lysate (RIPA lysis)
Lane 4: Rat cerebellum tissue lysate (hot lysis)

Lysates/proteins at 40 ug/Lane.

Predicted band size: 101 kDa
Observed band size: 110/60 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32384) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384]

Flow Cytometry: Glutamate Receptor 4 Antibody (JE36-81) [NBP3-32384] - Flow cytometric analysis of SH-SY5Y cells labeling Glutamate Receptor 4.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32384, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).