Glutathione S Transferase kappa 1 Antibody (7J5B3)

Name: Glutathione S Transferase kappa 1 Antibody (7J5B3)
Brand: Novus Biologicals
SKU: NBP3-16857
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16857

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7J5B3 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Glutathione S Transferase kappa 1 (Q9Y2Q3). MGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutathione S Transferase kappa 1 Antibody (7J5B3)

Western Blot: Glutathione S Transferase kappa 1 Antibody (7J5B3) [NBP3-16857]

Western Blot: Glutathione S Transferase kappa 1 Antibody (7J5B3) [NBP3-16857] - Western blot analysis of extracts of various cell lines, using Glutathione S Transferase kappa 1 Rabbit mAb (NBP3-16857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: Glutathione S Transferase kappa 1 Antibody (7J5B3) [NBP3-16857]

Immunocytochemistry/Immunofluorescence: Glutathione S Transferase kappa 1 Antibody (7J5B3) [NBP3-16857] - Immunofluorescence analysis of NIH-3T3 cells using Glutathione S Transferase kappa 1 Rabbit mAb (NBP3-16857) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Glutathione S Transferase kappa 1 Antibody (7J5B3)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione S Transferase kappa 1

Glutathione transferases (GSTs) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione (GSH) to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells (1) The Kappa class of GSTs comprises soluble enzymes originally isolated from the mitochondrial matrix of rats. Gsk1 catalyses some typical GST reactions, it has been proposed that it is structurally distinct from other classes of cytosolic GSTs (2). Gsk1 has been identified in rat, mouse, and human. The C terminus of Gsk1 was essential for localization of the protein to peroxisomes, and the C-terminal sequence Ala-Arg-Leu represents a peroxisome targeting signal (3).

Alternate Names

EC 2.5.1.18, glutathione S-transferase k1, glutathione S-transferase kappa 1, Glutathione S-transferase subunit 13, glutathione S-transferase subunit 13 homolog, GST, GST 13-13, GST class-kappa, GST13, GST13-13, GSTK1-1, hGSTK1

Gene Symbol

GSTK1

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Certificate of Analysis

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Product Specific Notices for Glutathione S Transferase kappa 1 Antibody (7J5B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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