GM130/GOLGA2 Antibody (M342)

Name: GM130/GOLGA2 Antibody (M342)
Brand: Novus Biologicals
SKU: NBP3-23353
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23353

Submit a review

Citations (1)

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # M342

View all GM130/GOLGA2 Primary Antibodies »

Product Specifications

Immunogen

Clone M342 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human GM130/GOLGA2. This sequence has high homology to similar regions in rat and mouse GM130/GOLGA2. [UniProt# Q08379]

Specificity

This antibody detects a 130 kDa protein corresponding to the apparent molecular mass of GM130/GOLGA2 on SDS-PAGE immunoblots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for GM130/GOLGA2 Antibody (M342)

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody (M342) [NBP3-23353] -

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Immunocytochemical labeling of GM130/GOLGA2 in C2C12 cells. The cells were double-labeled with GM130/GOLGA2 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.

Western Blot: GM130/GOLGA2 Antibody (M342) [NBP3-23353] -

Western Blot: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Western blot analysis of GM130/GOLGA2 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130/GOLGA2 (C-terminal region) at 1:500.

Applications for GM130/GOLGA2 Antibody (M342)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: GM130/GOLGA2

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Gene Symbol

GOLGA2

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody (M342)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GM130/GOLGA2 Antibody (M342)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Inflammatory Mediators

Citations for GM130/GOLGA2 Antibody (M342)

Customer Reviews for GM130/GOLGA2 Antibody (M342)

There are currently no reviews for this product. Be the first to review GM130/GOLGA2 Antibody (M342) and earn rewards!

Have you used GM130/GOLGA2 Antibody (M342)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image