GOLGA5 Antibody (6B3) - Azide and BSA Free

Novus Biologicals | Catalog # H00009950-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6B3

Format

Azide and BSA Free
View all GOLGA5 Primary Antibodies »

Product Specifications

Immunogen

GOLGA5 (NP_005104, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SWFVDLAGKAEDLLNRVDQGAATALSRKDNASNIYSKNTDYTELHQQNTDLIYQTGPKSTYISSAADNIRNQKATILAGTANVKVGSRTPVEASHPVE

Reactivity Notes

Human. Other species not tested.

Marker

Golgi Apparatus Marker

Specificity

GOLGA5 (6B3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for GOLGA5 Antibody (6B3) - Azide and BSA Free

Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01]

Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01]

Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01] - GOLGA5 monoclonal antibody (M01), clone 6B3 Analysis of GOLGA5 expression in K-562.
Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01]

Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01]

Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01] - Analysis of GOLGA5 over-expressed 293 cell line, cotransfected with GOLGA5 Validated Chimera RNAi ( Cat # H00009950-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GOLGA5 monoclonal antibody (M01), clone 6B3 (Cat # H00009950-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01]

Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01]

Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01] - Analysis of GOLGA5 expression in transfected 293T cell line by GOLGA5 monoclonal antibody (M01), clone 6B3.Lane 1: GOLGA5 transfected lysate(83 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: GOLGA5 Antibody (6B3) [H00009950-M01] - Detection limit for recombinant GST tagged GOLGA5 is approximately 0.1ng/ml as a capture antibody.

Applications for GOLGA5 Antibody (6B3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GOLGA5

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5.

Alternate Names

Cell proliferation-inducing gene 31 protein, golgi autoantigen, golgin subfamily a, 5, golgin A5, Golgin subfamily A member 5, golgin-84, GOLIM5, Protein Ret-II, PTC5, RET-fused gene 5 protein, RETII, ret-II, rfg5, RFG5golgi integral membrane protein 5

Entrez Gene IDs

9950 (Human)

Gene Symbol

GOLGA5

OMIM

188550 (Human)

UniProt

NP_005104 (Human)

Additional GOLGA5 Products

Product Documents for GOLGA5 Antibody (6B3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GOLGA5 Antibody (6B3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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