GPR109B/HM74 Antibody - BSA Free

Novus Biologicals | Catalog # NLS179

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPR109B/HM74 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 18 amino acid peptide from N-terminal extracellular domain of human GPR109B/HM74.

Epitope

N-Terminus extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%).

Localization

Multi-pass membrane protein

Specificity

Human GPR109B/HM74. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HCAR2 (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for GPR109B/HM74 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPR109B/HM74 Antibody - BSA Free [NLS179]

Immunohistochemistry-Paraffin: GPR109B/HM74 Antibody - BSA Free [NLS179]

Immunohistochemistry-Paraffin: GPR109B/HM74 Antibody [NLS179] - Analysis of anti-GPR109B / HM74 antibody with human adipocytes at dilution 3-4 ug/ml.
Immunohistochemistry-Paraffin: GPR109B/HM74 Antibody - BSA Free [NLS179]

Immunohistochemistry-Paraffin: GPR109B/HM74 Antibody - BSA Free [NLS179]

Immunohistochemistry-Paraffin: GPR109B/HM74 Antibody [NLS179] - Analysis of anti-GPR109B / HM74 antibody with human spleen.

Applications for GPR109B/HM74 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

3 - 4 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 17008871)..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: GPR109B/HM74

HM74 acts as a low affinity receptor for nicotinic acid and mediates its anti-lipolytic effect through a G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Expression in humans is largely restricted to adipose tissue and spleen but there are some reports of it being expressed in lymphocytes and monocytes. In mouse is expressed in lungs, spleen, heart, skeletal muscle and adipose tissue. In rat it is expressed in adipose tissue, lung and spleen. ESTs have been isolated from adipose, blood, liver, liver/spleen, placenta, prostate, and testis libraries.

Long Name

G Protein-coupled Receptor HM74 Protein-coupled Receptor 109B

Alternate Names

HCA3, HCAR3, HM74, NIACR2

Entrez Gene IDs

8843 (Human)

Gene Symbol

HCAR3

UniProt

P49019 (Human)

Additional GPR109B/HM74 Products

Product Documents for GPR109B/HM74 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GPR109B/HM74 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GPR109B/HM74 Antibody - BSA Free

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Protocols

View specific protocols for GPR109B/HM74 Antibody - BSA Free (NLS179):

Immunohistochemistry Protocol for HM74 Antibody (NLS179):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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