Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPR119 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human GPR119 protein (within residues 150-250). [Swiss-Prot Q8TDV5]

Reactivity Notes

Immunogen sequence has 88% identity to rat.

Localization

Cell membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit GPR119 Antibody - BSA Free (NLS548) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GPR119 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GPR119 Antibody - BSA Free [NLS548]

Immunocytochemistry/ Immunofluorescence: GPR119 Antibody - BSA Free [NLS548]

Immunocytochemistry/Immunofluorescence: GPR119 Antibody [NLS548] - GPR119 antibody was tested in MCF-7 cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: GPR119 Antibody - BSA Free [NLS548]

Immunohistochemistry: GPR119 Antibody - BSA Free [NLS548]

Immunohistochemistry: GPR119 Antibody [NLS548] - Staining of human brain, neuron.
Immunohistochemistry: GPR119 Antibody - BSA Free [NLS548]

Immunohistochemistry: GPR119 Antibody - BSA Free [NLS548]

Immunohistochemistry: GPR119 Antibody [NLS548] - Analysis of GPR119 in mouse pancreas using DAB with hematoxylin counterstain.
Immunohistochemistry: GPR119 Antibody - BSA Free [NLS548]

Immunohistochemistry: GPR119 Antibody - BSA Free [NLS548]

Immunohistochemistry: GPR119 Antibody [NLS548] - Analysis of GPR119 in human pancreas using Fast Red with hematoxylin counterstain.

Applications for GPR119 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100
Application Notes
This GPR119 antibody is useful for Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPR119

GPR119 (G-protein coupled receptor 119) is a class A (rhodopsin-like) 7-transmembrane GPCR with little homology to other receptors, and human GPR119 receptor has 335 amino acids which is highly conserved in mouse and rat. GPR119 is expressed in human insulin-secreting pancreatic islets and incretin (GIP and GLP-1) secreting K- and L-cells of gastrointestinal tract. GPR119 has been suggested to function as a glucose-dependent insulinotropic receptor and its activity is mediated by G proteins which activate adenylate cyclase. Lysophosphatidylcholine (LPC), oleoylethanolamide (OEA), N-acylated ethanolamine phospholipids (NAPEs) and N-oleoyldopamine (OLDA), and 5-hydroxyeicosapentaenoic acid (5-HEPE) have all been proposed as GPR119's endogenous ligands. Moreover, GPR119 agonists have been shown to elicit nutrient-independent incretin and gastrointestinal hormone release as well as nutrient-stimulated insulin secretion, thereby lowering plasma glucose levels during an oral glucose tolerance test. In rodent models, GPR119 mediates improved glucose tolerance and prevents weight gain. GPR119 agonism increases the release of incretin hormones and insulin from enteroendocrine and pancreatic beta cells which may underlie these beneficial effects. GPR119 agonists play a role in reducing food intake and promoting weight loss, possibly through the release of GLP-1, which is known to delay gastric emptying.

Long Name

G Protein-coupled Receptor 119

Alternate Names

GPCR2

Entrez Gene IDs

139760 (Human); 236781 (Mouse)

Gene Symbol

GPR119

UniProt

Q8TDV5 (Human), Q7TQP3 (Mouse)

Additional GPR119 Products

Product Documents for GPR119 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GPR119 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for GPR119 Antibody - BSA Free (NLS548):

GPR119 Antibody:
Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4-mm sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.
**Do not allow the tissues to dry at any time during the staining procedure**
12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.
**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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