GRAP2 Antibody (OTI1G2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71457

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G2

Format

Azide and BSA Free
View all GRAP2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GRAP2(NP_004801) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRAP2 Antibody (OTI1G2) - Azide and BSA Free

Western Blot: GRAP2 Antibody (OTI1G2)Azide and BSA Free [NBP2-71457]

Western Blot: GRAP2 Antibody (OTI1G2)Azide and BSA Free [NBP2-71457]

Western Blot: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRAP2.
Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GRAP2 mouse monoclonal antibody.
Flow Cytometry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Flow Cytometry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Flow Cytometry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GRAP2 antibody, and then analyzed by flow cytometry.
Western Blot: GRAP2 Antibody (OTI1G2)Azide and BSA Free [NBP2-71457]

Western Blot: GRAP2 Antibody (OTI1G2)Azide and BSA Free [NBP2-71457]

Western Blot: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRAP2 monoclonal antibody.
Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody.
Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Immunohistochemistry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457] - Staining of paraffin-embedded Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody.
Flow Cytometry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Flow Cytometry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457]

Flow Cytometry: GRAP2 Antibody (OTI1G2) - Azide and BSA Free [NBP2-71457] - Analysis of Jurkat cells, using anti-GRAP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for GRAP2 Antibody (OTI1G2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRAP2

GRAP2 encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.

Long Name

GRB2-related Adaptor Protein 2

Alternate Names

GADS, GRB2L, GRBLG, GRBX, Grf40, GRID, GRPL, Mona

Gene Symbol

GRAP2

Additional GRAP2 Products

Product Documents for GRAP2 Antibody (OTI1G2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GRAP2 Antibody (OTI1G2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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