GREB1 Antibody (CL13863) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43840

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13863

Format

Azide and BSA Free
View all GREB1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q4ZG55, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GREB1 Antibody (CL13863) - Azide and BSA Free

GREB1 Antibody (CL13863) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: GREB1 Antibody [NBP3-43840]

Immunocytochemistry/ Immunofluorescence: GREB1 Antibody [NBP3-43840]

Staining in U2OS cell line with Anti-GREB1 monoclonal antibody).
GREB1 Antibody (CL13863) - Azide and BSA Free Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
GREB1 Antibody (CL13863) - Azide and BSA Free Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Staining of human fallopian tube shows weak to moderate cytoplasmic and nuclear positivity in glandular cells.
GREB1 Antibody (CL13863) - Azide and BSA Free Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Staining of human ovarian cancer (sex cord tumor) shows strong nuclear positivity in tumor cells.
GREB1 Antibody (CL13863) - Azide and BSA Free Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Staining of human ovarian cancer (granulosa cell tumor) shows moderate nuclear positivity in tumor cells.
GREB1 Antibody (CL13863) - Azide and BSA Free Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Staining of human prostate cancer shows strong nuclear positivity in tumor cells.
GREB1 Antibody (CL13863) - Azide and BSA Free Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Immunohistochemistry-Paraffin: GREB1 Antibody [NBP3-43840]

Staining of human small intestine shows no positivity in glandular cells as expected.

Applications for GREB1 Antibody (CL13863) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For Immunocytochemistry/ Immunofluorescence/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GREB1

GREB1 is an estrogen-responsive gene that is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (a) encodes the longest isoform (a).

Alternate Names

gene regulated by estrogen in breast cancer protein, growth regulation by estrogen in breast cancer 1, KIAA0575Gene regulated in breast cancer 1 protein, protein GREB1

Gene Symbol

GREB1

Additional GREB1 Products

Product Documents for GREB1 Antibody (CL13863) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GREB1 Antibody (CL13863) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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